Addiction Research

Abstract

Dopamine D1 and D2 Receptor Subtypes Functional Regulation in Unilateral Rotenone Lesioned Parkinson’s Rat Model: Effect of Serotonin, Dopamine and Norepinephrine

Jes Paul, Nandhu M.S, Korah P. Kuruvilla and Paulose C.S

Parkinson’s disease is a progressive neurodegenerative disorder characterized by selective degeneration of dopaminergic neurons in substantia nigra pars compacta leading to marked reduction of dopamine levels in the cerebral cortex. The present study analysed the effect of serotonin, dopamine and norepinephrine as treatment on rotenone induced hemi-Parkinson’s disease in rats and its role in the regulation of Dopamine receptor subtypes in the cerebral cortex of the experimental rats. Unilateral stereotaxic single dose infusions of rotenone were administered to the substantia nigra of adult male Wistar rats. Neurotransmitters –serotonin, dopamine and norepinephrine treatments were given to rotenone induced hemi-Parkinson’s rats. Scatchard analysis of Dopamine D1 and D2 receptor showed a significant increase (p<0.001) in the cerebral cortex of the Parkinson’s rats compared to control. These altered parameters were reversed to near control in the serotonin and norepinephrine treated Parkinson’s disease rats and no change was observed in Dopamine treated Parkinson’s disease rats. Real-time PCR results confirmed the receptor data. Cognitive and sensorimotor activities were reduced in Parkinson’s disease rats which were reversed by serotonin and norepinephrine. Our results showed serotonin and norepinephrine functionally
reversed the Dopamine receptors significantly in rotenone induced hemi-Parkinson’s rat. This has clinical significance in the therapeutic management of Parkinson’s disease.